Strategies for Attaching Oligonucleotides to Solid Supports
نویسندگان
چکیده
While most molecular reactions involving synthetic oligonucleotides, notably PCR and DNA sequencing, are carried out in liquid phase, a rapidly growing number of important applications utilize synthetic oligonucleotides attached to solid supports. In general, the purpose for using a solid support-attached oligonucleotide is to capture something, hold on to it, enrich it, and identify and/or purify it. The major class of solid support-attached oligonucleotides, DNA oligonucleotide microarrays, provide a powerful tool for analysis of gene expression. The most generally accessible approach for producing oligonucleotide microarrays is to synthesize the individual oligonucleotides separately prior to immobilization on the solid surface. In this case the oligonucleotide is modified with a functional group that allows attachment to a reactive group on a solid surface. Another important class is the micro-bead-attached oligonucleotide.
منابع مشابه
Strategies for Attaching Oligonucleotides to Solid Support
Many important molecular applications, such as DNA oligonucleotide arrays, utilize synthetic oligonucleotides attached to solid supports. The most accessible approach for producing an oligonucleotide microarray is to synthesize individual oligonucleotides and subsequently immobilize them to a solid surface. For this immobilization to take place, the oligonucleotides must be modified with a func...
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